Fig. 1

Expression of PABPC1 in ESCC tissues with its clinical significance. A Different expression levels of RNA-binding proteins, between normal and ESCC tissues, based on TCGA (https://cancergenome.nih.gov/). B Expression of PABPC1 in different types of cancer in the GEPIA database (http://gepia.cancer-pku.cn/). C Expression of PABPC1 in 24 pairs of ESCC samples (T) and corresponding non-tumor tissues (N) was detected by western blot analysis. D PABPC1 mRNA expression was detected by qRT-PCR in ESCC samples and non-tumor tissues (n = 48) and in TCGA. E Immunohistochemical staining of PABPC1 in primary ESCC samples and non-tumor tissues (n = 190) (scale bars: 200 µm, 50 µm). F Immunohistochemical staining of PABPC1 and CD34 in primary ESCC samples (n = 190) (scale bars: 200 µm). G Expression of PABPC1 in the immortalized esophageal cell line NE1 and ESCC cell lines (KYSE150, KYSE520, KYSE510, KYSE410, KYSE450, KYSE180, 81 T, and TE1) was detected by qRT-PCR and western blotting. H Expression of PABPC1 in ESCC cell lines KYSE150 and KYSE520 was detected by immunofluorescence (scale bars: 20 µm). I Expression of PABPC1 was higher in poorly differentiated than well differentiated tissues. J Comparison of overall survival of ESCC patients with high and low PABPC1 protein expression was determined by Kaplan–Meier survival analysis. Data represent the mean ± SD of 3 separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test