Fig. 2

P300/Sp1 upregulates PABPC1 expression by modulating PABPC1 promoter H3K27 acetylation. A Expression of PABPC1 was detected by western blotting and qRT-PCR following treatment of KYSE150 and KYSE520 cells with different doses of NaBu. B ChIP assay demonstrating that H3K27 acetylation occurred in the PABPC1 promoter in ESCC cell lines following treatment with NaBu (2 mM). C ChIP assay demonstrating that H3K27 acetylation occurred in the PABPC1 promoter in the immortalized esophageal cell line NE1 and KYSE150 and KYSE520 ESCC cell lines. ChIP assay demonstrating that H3K27 acetylation occurred in the PABPC1 promoter in normal and ESCC tissues (n = 3). D Expression of PABPC1 was detected by western blotting and qRT-PCR following treatment of KYSE150 and KYSE520 cells with C646 or A485. E ChIP assay demonstrating that H3K27 acetylation occurred in the promoter of PABPC1 in ESCC cell lines following transfection of p300. F Expression of PABPC1 was detected after co-transfection of Sp1 and p300 plasmids and detection by western blot and qRT-PCR. G HEK293T cells were co-transfected with different combinations of wild-type (wt) and Sp1-site-mutated reporter constructs (mt 1, mt 2, and mt 3), p300 and control. The relative luciferase activity was analyzed. H Correlation of PABPC1 and Sp1 or p300 in TCGA was analyzed. Data represent the mean ± SD of 3 separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test