Fig. 3

PABPC1 promotes cell proliferation, migration, and invasion in vivo and in vitro. A Confirmation of PABPC1 plasmid and siRNA transfection by western blot and qRT-PCR. B Cell invasion and migration was detected by transwell assay in PABPC1-overexpressing or knockdown ESCC cell lines (scale bar: 200 µm). C Cell migration was detected by wound healing assay in PABPC1-overexpressing or knockdown ESCC cell lines (scale bar: 200 µm). D Capillary tube formation assay of HUVECs co-cultured with PABPC1-overexpressing or knockdown ESCC cells. E Effects of PABPC1 overexpression on ESCC progression in subcutaneous xenograft mouse models. Tumor growth volume and weight of xenografts derived from control or PABPC1 cells are shown. F Representative photographs of immunohistochemical staining of tumor tissues from mice inoculated with PABPC1-overexpressing and control cells (scale bar: 200 µm). G Representative images of mass and H&E-stained sections from metastatic nodules in the lung (scale bar: 200 µm). Data represent the mean ± SD of 3 separate determinations. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test