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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma

Fig. 6

Targeted knockdown of Rad18 based on exosome delivery and RNAi technology increases the sensitivity of osteosarcoma cells to doxorubicin. A Schematic image. Process of RGD-EXO construction, isolation, siRad18 loading, animal tail vein injection. B Left, NIR fluorescence imaging of mice at 0 h, 3 h, 6 h, 12 h, and 24 h post-injection with DID-stained RGD-EXOs or Exosome, which loaded cholesterol-cy3-siRad18. Right, biodistribution of DID-stained RGD-EXOs and Exosome were tracked 24 h after IV injection. C Quantitative analysis of NIR fluorescence signal from nude mice that received cholesterol-cy3-siRad18 loaded and DID stained RGD-EXOs and Exosome. D Time line of nude mice receiving combination therapy. E and F Representative images and data analysis of animal bioluminescence at different time points in each treatment group. G and H Weight and appearance of OS in vitro after the last treatment. I Representative images of H&E and Rad18, γ-H2AX and cleaved Caspase-3 IHC in OS sections of indicated groups. Scale bar =50 μm

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