Fig. 3From: Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progressionYBX1 protein binds hY4F to regulate its sorting into lung cancer EVs. A Schematic representation of the biotin-labelled hY4F RNA pulldown assay. B Representative MS/MS data of specific peptides of YBX1 identified in this paper. C) Western blot analysis of hY4F-binding proteins enriched by the RNA pulldown assay. D Western blot analysis of YBX1 and Ro60 in IMR-90 and A549 cells. E Western blot analysis of siRNA-mediated YBX1 knockdown of A549 cells after transfection for 24 h. And #1, #2, and #3 means different siRNAs targeting YBX1. F Quantitative PCR results of hY4F in EVs and cell lysates from A549 transfected with siRNA (#1) targeting YBX1 for 48 h. G Quantitative PCR results of hY4F in EVs and cell lysates from wild type and YBX1 knockout (KO) A549 cells. H Quantitative PCR data of hY4F in EVs from A549-YBX1 KO cells overexpressing YBX1 or an empty vector. I Quantitative data of wild type and YBX1-KO A549 EVs using the surface protein BCA assay. Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001. ns: no significant difference (Student’s t-test). NC: negative controlBack to article page