Fig. 4

YBX1 promotes lung cancer cell proliferation and migration. A CCK-8 (at 24, 48, 72 and 96 h after transfection) and (B) EdU assays (at 48 h after transfection) were performed to assess the effect of YBX1 knockdown and over-expressing on A549 cell proliferation. C Flow cytometry using Annexin V-FITC/PI staining was performed to analysis apoptosis of YBX1 knockdown and overexpressing A549 cells (at 24 h after transfection). D Colony formation assay with A549 cells at 7 d after knockdown and overexpression of YBX1.The effect of YBX1 knockdown and overexpression on A549 (E) migration and (F) invasion was examined by transwell assay (at 24 h after transfection). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative control