Fig. 8

YBX1 mediated regulation of hY4F sorting into EVs is necessary for the proliferation and metastasis of lung cancer cells in vivo. A The growth curves of tumor volumes for xenotransplantation nude mice subcutaneously injected with WT/YBX1-KO A549 (n = 7). Photographs (B) and weights (C) of subcutaneous tumors at day 34 of tumor growth are shown. D Quantitative PCR results of hY4F in plasma from nude mice subcutaneously injected with WT/YBX1-KO A549. E Representative photos and (F) H&E stain results of lungs from nude mice injected WT/YBX1-KO A549 cells (n = 6) via tail vein for 5 weeks. Blue arrows indicate the tumor metastasis. G The statistical results of the number and area of lung metastatic lesions formed by A549 cells xenograft according to H&E stain images. H Levels of YBX1 protein in tissue from LUAD cancer patients or control subjects according to the CPTAC database (p < 0.001). The grades (I) and stages (J) of LUAD cancer patients are closely associated with YBX1 protein levels in cancer tissue according to the CPTAC database (Grade1 vs control: p < 0.01, Grade2/3 vs control: p < 0.001; Stage1/2/3 vs normal: p < 0.001). K Overall survival rate of LUAD cancer patients with high or low YBX1 mRNA level in tissue from LUAD cancer patients according to the TCGA database. L Overall survival rate of LUAD cancer patients with high or low hY4 RNA (RNY4) expression levels in tissue according to the TCGA database. M Correlational analysis of hY4F and YBX1 levels in LUAD patients according to the TCGA database. Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). WT: wild type; KO: knockout