Fig. 2

Ectopic overexpression of IL-32 enhances ESCC cells migration and invasion. A, B The expression level of IL-32 in EC109 shIL-32, EC109 shNC, KYSE150 IL-32β and KYSE150 vector cell lines was detected by qRT-PCR and Western blot assay. Transwell (C, D), Wound healing (E, F) and Invasion (G, H) assays were performed using EC109 shNC, EC109 shIL-32 KYSE150 vector and KYSE150 IL-32β cell lines. Five fields were included per assay, and the experiment was repeated three times. Scale bar, 100 or 200 μm. All data are represented as means ± SEM. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001