Fig. 2

mMSCs-Exo attenuated radiation-induced EMT in MLE-12 cells. A Representative images of cell morphology at 48 h after a single dose of 8 Gy radiation or non-radiation. Zoom-in images were shown to closely observe the epithelial or mesenchymal morphology. Arrows represent extended pseudopodia (black) and elongated appearance (red). Scale bars = 100 μm. B, C Western Blot analysis (B) and densitometric quantification (C) of E-cadherin, Vimentin and GAPDH (for normalization) in nonirradiated control and irradiated cells with or without mMSCs-Exo. D Immunofluorescence staining for E-cadherin, Vimentin (green), and DAPI (blue) in nonirradiated control and irradiated cells with or without mMSCs-Exo. Scale bars = 100 μm. Data are presented as the mean ± SD. from three independent experiments, * p < 0.05