Fig. 4
Lsm12 promotes the cell proliferation, mobility and tumorigenicity in vivo. a Cell growth curve analysis showed that the growth rate of OSCC cells was significantly increased when Lsm12 was overexpressed and decreased upon Lsm12 knockdown. b, c Colony formation assay revealed that colony formation ability of OSCC cells was significantly increased when Lsm12 was overexpressed and decreased upon Lsm12 knockdown. d-f Cell cycle analysis showed that Lsm12 overexpression increased the proportions of S phase and G2/M phase but Lsm12 knockdown decreased the proportion of S phase significantly in OSCC cells. g-i Transwell assay showed the effect of Lsm12 on the migration and invasion abilities of OSCC cells. j The results of in vivo tumorigenicity assay showed that no tumor formation was observed in five mice of Lsm12 knockdown group and the tumors were formed in five mice of the control group. Histopathological examination of the excised tumors showed squamous cell carcinoma. Bar, 50 μm. *, ** and *** indicate P < 0.05, P < 0.01 and P < 0.001, respectively