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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Identification of RNA-splicing factor Lsm12 as a novel tumor-associated gene and a potent biomarker in Oral Squamous Cell Carcinoma (OSCC)

Fig. 6

Lsm12 regulates alternative splicing of USO1. a USO1 gene has two transcript variants according to UCSC database. Alternative splicing of USO1 exon 15 is shown. b Whole transcriptome sequencing analysis revealed that Lsm12 knockdown induced USO1 exon 15 skipping. c-f The results of PCR assay and sequencing showed that Lsm12 regulated the alternative splicing of USO1 exon 15 in SCC-25 cells and modulated the expression level of USO1 in CAL 27 cells. Red arrows indicate exon 15 skipping. g, h PCR assay and sequencing showed that there were two USO1 transcript variants with or without exon 15 in SCC-25 cells, but only one USO1 transcript variant with exon 15 was observed in CAL 27 cells. USO1 exon 5 was found constitutively to be skipped in both SCC-25 and CAL 27 cells. Blue arrows indicate Exon 5 skipping

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