Fig. 7
USO1 containing exon 15 promotes cell proliferation, mobility and tumorigenicity in vivo. a The results of PCR assay and sequencing showed that the stable cell lines overexpressing USO1 with or without exon 15 were constructed in CAL 27 cells. b-e Overexpression of USO1 containing exon 15 significantly promoted the cell proliferation, increased the proportion of G2/M phase, and enhanced the colony formation, cell migration and invasion abilities of OSCC cells. f 3.5 × 106 USO1-FL or USO1-DE cells were subcutaneously injected into each of six nude mice. Excised tumors were shown. Tumor volume and weight were increased remarkably in the group of USO1 with exon 15, compared with those in the group of USO1 without exon 15. Histopathological examination of the tumor specimens from these two groups showed squamous cell carcinoma. Bar, 50 μm