Fig. 2

miR-15b-5p transferred by M2 macrophage-derived EVs to GC cells where it promotes GC cell metastasis. A Uptake of M2 macrophage-derived EVs by AGS and MKN-45 cells observed under a fluorescence microscope (scale bar = 25 μm). B pCDNA3.1-GFP-transfected AGS and MKN-45 cells following co-culture with the miR-15b-5p-Cy3-transfected M2 macrophages observed under a confocal microscope (scale bar = 25 μm). C Expression of miR-15b-5p determined by RT-qPCR in M2 macrophages and the isolated EVs. D Expression of miR-15b-5p determined by RT-qPCR in AGS and MKN-45 cells treated with EV-mimic-NC or EV-miR-15b-5p-mimic. E Colony formation of GC cells in the presence of EV-mimic-NC or EV-miR-15b-5p-mimic. F Invasion of GC cells measured by Transwell assay in the presence of EV-mimic-NC or EV-miR-15b-5p-mimic. G Matrix adhesion ability of AGS and MKN-45 cells in the presence of EV-mimic-NC or EV-miR-15b-5p-mimic. H Western blot analysis of E-cadherin, N-cadherin, and Vimentin proteins in AGS and MKN-45 cells in the presence of EV-mimic-NC or EV-miR-15b-5p-mimic. Each experiment was conducted three times independently. * p < 0.05, compared with AGS and MKN-45 cells treated with mimic-NC, PBS, mimic-NC + Vector. # p < 0.05, compared with AGS and MKN-45 cells treated with EV-mimic-NC, miR-15b-5p mimic + Vector