Fig. 3

BRMS1 is a target of miR-15b-5p in GC cells. A A volcano plot of differentially expressed genes obtained from the GC-related GSE49051 dataset. B BRMS1 expression in the GSE49051 dataset. Red indicates GC samples and gray indicates normal samples. C Relationship between BRMS1 expression and survival of patients with GC, analyzed using the GEPIA2 database (http://gepia2.cancer-pku.cn/#index). D Expression of BRMS1 determined by RT-qPCR in GC tissues and adjacent normal tissues from 49 patients with GC. E Expression of BRMS1 determined by RT-qPCR in GC cell lines HGC-27, SNU-1, AGS and MKN-45, and normal gastric epithelial cell line GES-1. F Predicted miR-15b-5p binding sites in the 3’UTR of BRMS1 using the starBase database. G Binding of miR-15b-5p to BRMS1 confirmed by dual-luciferase reporter assay. H miR-15b-5p expression determined by RT-qPCR in AGS ad MKN-45 cells transfected with miR-15b-5p inhibitor. I Western blot analysis of BRMS1 protein in AGS and MKN-45 cells transfected with miR-15b-5p mimic or inhibitor. Each experiment was conducted three times independently. * p < 0.05, compared with adjacent normal tissues, GES-1 cell line, 293 T cells or AGS and MKN-45 cells transfected with mimic-NC. # p < 0.05, compared with AGS and MKN-45 cells transfected with inhibitor-NC