Fig. 5

BRMS1 positively correlated with DAPK1 in GC cells. A Expression of DAPK1 obtained from the GC-related GSE49051 dataset. Red indicates normal samples and gray indicates GC samples. B Expression of DAPK1 determined by RT-qPCR in adjacent normal and GC tissues from 49 patients with GC. C Expression of BRMS1 determined by RT-qPCR in GC cell lines HGC-27, SNU-1, AGS, and MKN-45, and normal gastric epithelial cell line GES-1. D Relationship between BRMS1 expression and DAPK1 expression analyzed by Pearson's correlation coefficient. E BRMS1 binding sites in the DAPK1 promoter region and mutation sequences of DAPK1. F Enrichment of BRMS1 in the DAPK1 promoter region in AGS and MKN-45 cells analyzed by ChIP. G BRMS1 binding to DAPK1 confirmed by dual-luciferase reporter assay in 293 T cells. H BRMS1 expression determined by RT-qPCR in AGS and MKN-45 cells treated with sh-BRMS1-1 and sh-BRMS1-2. I Western blot analysis of BRMS1 and DAPK1 proteins in AGS and MKN-45 cells sh-BRMS1 or BRMS1. Each experiment was conducted three times independently. * p < 0.05, compared with adjacent normal tissues, GES-1 cell line, IgG or AGS and MKN-45 cells treated with sh-NC. # p < 0.05, compared with 293 T cells or AGS and MKN-45 cells treated with Vector