Fig. 3

circZNF609 depletion drives melanoma metastasis in vitro. a Schematic illustration showing three targeted siRNAs. b Left, qRT-PCR analysis of circZNF609 and ZNF609 mRNA in LM-MEL-45 cells treated with siRNAs. Right, circZNF609 overexpression efficiency in LM-MEL-45 cells. c Cell migratory and invasive capabilities were assessed by Transwell assay after knocking down of circZNF609 in 2 acral melanoma cells and 2 cutaneous melanoma cells. d Cell migratory capability was assessed by wound healing assay after knocking down or overexpressing circZNF609 in 2 melanoma cells. e Cell invasive capability was assessed by 3D spheroid-based Matrigel invasion assay after knocking down or overexpressing circZNF609 in LM-MEL-45 cells