Fig. 4

Ectopic expression of circZNF609 suppresses melanoma metastasis in vivo. a-d Representative bioluminescent images (a), Kaplan-Meier survival analysis (b), H&E staining (c) and quantification (d) of lung metastatic colonization of nude mice treated with tail-vein injection of firefly luciferase-expressing A2058 cells stably transfected with vector or circZNF609. e-g Representative images (e), quantification (f) and H&E staining (g) of lung metastatic colonization of NOD/SCID mice treated with tail-vein injection of HMY-1 cells stably transfected with vector or circZNF609. h-j Representative bioluminescent images (h), quantification (i) and H&E staining (j) of liver metastatic colonization of NOG mice treated with tail-vein injection of firefly luciferase-expressing HMY-1 cells stably transfected with vector or circZNF609. White arrows represent hemispleen