Fig. 3

citH3 levels are directly related to cfDNA levels and inversely related to cfmtDNA in EC sera. Box-plots of citH3 content in (A) HS and EC sera (B) G1, G2, and G3 EC grades. C Cluster analysis of citH3 levels in EC sera displaying a cfDNA content ≤ 712,3 or > 712, (D) cfmtDNA content ≤ 16,5 or > 16,5. Boxes extend from the 25th to 75th percentiles, the horizontal line in the box represent median. P values, Mann–Whitney test in panel A-C-D; Kruskall-Wallis test in B; n.s.: not significant. E Identification of EC-DNA-characteristics related to different grade. Plot of the two-dimension multiple corresponding analysis (MCA) among HSs and EC grades (G1, G2, G3) of cfDNA, cfmtDNA and citH3 categorized according to cut-offs individuated by ROC analysis