Fig. 7

Inhibitor-dependent effect on DNA damage and replication stress in MCF-7 and Y537S cells.Western blot and relative densitometric (G and H) analyses of ERα, RPA2 and phosphorylated γH2AX expression levels in MCF-7 (A, C, and E) and Y537S (B, D, and F) cells treated for 24 h with the indicated doses of the specific inhibitors of either CHK1 (i.e., MK8776—MK), CHK2 (i.e., CCT241533—CCT), ATR (i.e., VE822—VE) or ATM (i.e., KU60019—KU) as well as with AZD7762 (AZD). The loading control was done by evaluating vinculin expression in the same filter. Significant differences are given in the heatmaps (G and H) with red being a significant increase and green being a significant decrease with respect to control (0). Analyses were performed by using the unpaired two-tailed Student’s t-test. Data are the mean ± the standard deviations, and blots show representative images of three different experiments. Histograms relative to the heatmaps are available in supplementary Fig. 7