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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Clinically relevant CHK1 inhibitors abrogate wild-type and Y537S mutant ERα expression and proliferation in luminal primary and metastatic breast cancer cells

Fig. 8

Replication stress and X-rays dependent effect on ERα levels in MCF-7 and Y537S cells. Western blot and relative densitometric (C and F) analyses of ERα, RPA2 and γH2AX expression levels in MCF-7 (A-C) and Y537S (B-F) cells treated for 24 h with hydroxyurea (HU—200 mM), etoposide (ETO – 1 μM), camptothecin (CPT – 100 nM) or aphidicolin (Aph – 5 μM). For X-rays, cells were lysed 4 h after irradiation and analyzed by Western blotting. The loading control was done by evaluating vinculin expression in the same filter. Significant differences are given in the heatmaps (C and F) with red being a significant increase and green being a significant decrease with respect to control (CTR). Analyses were performed by using the unpaired two-tailed Student’s t-test. Data are the mean ± the standard deviations, and blots show representative images of three different experiments. Histograms relative to the heatmaps are available in supplementary Fig. 7

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