Fig. 2

The oncogenic effect of WASF2 overexpression in normal hepatocytes and the anti-tumorigenic effects of WASF2 knockdown in HCC cells. A Representative western blot of the Flag and WASF2 expression in MIHA cells transfected with Empty vector or different concentrations of EX-WASF2. B Left: Representative morphology and number of MIHA cells transfected with Empty vector or EX-WASF2. Scale bar = 100 μm. Cell number was counted using trypan blue (mean ± SEM, n = 3, unpaired t-test). Right: Cell growth was measured using MTT assay. (mean ± SEM, n = 3, unpaired t-test). C Clonogenic and D scratch wound healing assays were performed in MIHA cells transfected with Empty vector or EX-WASF2. (mean ± SEM; n = 3, unpaired t-test). Scale bar = 100 μm. E Growth analyses. Left: morphology and number of Huh-7 and SNU475 cells transfected with NC or siWASF2. Scale bar = 100 μm. Cell number was counted using trypan blue (mean ± SEM, n = 3, unpaired t-test). Right: MTT assay (mean ± SEM, n = 3, unpaired t-test). F Clonogenic and G scratch wound healing assay in Huh-7 and SNU475 cells. Clonogenic assay: left, representative colony images; right, graphical representation of colony number from three random images (mean ± SEM; n = 3). Wound healing assay: left, representative images; right, graphical representation of the percentage of migrated cells from three random images (mean ± SEM; n = 3, unpaired t-test). Scale bar = 10 μm. **P < 0.01; ***P < 0.001