Fig. 1

Hypoxia promoted macrophage-derived CXCL8 secretion. A, B Differential genetic analysis of the transcription data of macrophages cultured under hypoxia and normoxia showed that hypoxia could promote CXCL8 expression. (A. volcano; B. heatmap). C MACS obtained human CD14⁺ monocytes and induced them into macrophages through M-CSF. D, E Hypoxia promoted human-derived macrophage CXCL8 expression at both transcription and protein levels. F. Hypoxia promoted the secretion of macrophage-derived cytokine CXCL8 secretion. G An evident co-localization of macrophages and CXCL8 in human GC tissue specimens. H Schematic of gating strategy of flow cytometry analysis. GC tissue were dissociated to obtain a single-cell suspension and stained with antibodies. Cells were first gated to exclude debris and dead cells (Sup Fig. 1D), then GC cells and macrophages were selected. Cells were further gated by cluster of CXCL8 expression. I GC cells (green) and macrophages (red) were co-cultured under hypoxia to mimics the microenvironment. J CXCL8 was mainly derived from macrophages and IL-10 was expressed by GC cells in the co-culture system