Fig. 6

BGA002-RA treatment leads to autophagy reactivation in MNA-NB. A stacked bar-plot with the vertical axis representing the number of lysosomes per cell after 24 hours of treatment in the Kelly MNA-NB cell line (MYCN amplified, p53 wild type). The horizontal axis is the area range of the lysosomes. Colors represents treatment (control, 13-cis RA, BGA002, BGA002 + RA). B single bar plot for each treatment group is represented. As described previously, vertical axis represents the number of lysosomes and horizontal axis represents the area range of lysosomes. Only lysosomes considered with high dimension (> 2 μm) are represented in each graphs. Kelly treated with combination of BGA002 and 13-cis RA shows lysosomes with the highest diameters compared with other treatment group (two-sided unpaired test-T p < 0.01). BGA002 alone show only small differences compared with control (p = 0.053) and RA alone shows no differences