Fig. 6

circ_0087429 downregulates the expression of OGN through competitive binding with miR-5003-3p in cervical cancer. a. The databases miRDB, miRWalk, mirDIP and TargetScan predicted the target genes of miR-5003-3p b. The expression changes in OGN after circ_0087429 overexpression and miR-5003-3p knockdown were determined by qRT-PCR. c. The expression changes in OGN after circ_0087429 knockdown and miR-5003-3p overexpression were determined by qRT-PCR. d. The expression of OGN was detected by western blotting after the expression of circ_0087429 and miR-5003-3p was changed. e. The fluorescence intensity of OGN after circ_0087429 overexpression or knockdown was confirmed by immunofluorescence staining. Scale bar, 25 μm. f. The binding sites of circ_0087429 and miR-5003-3p were predicted by miRWalk. g. A luciferase reporter assay was used to examine the relative luciferase activity of OGN-WT and OGN-MUT. h-i. The differential expression of OGN between cervical cancer and normal cervical epithelial cell lines was detected by western blotting and qRT-PCR. j-k. The differential expression of OGN between cervical cancer (T) and adjacent tissue (P) samples was detected by western blotting and qRT-PCR. l. Spearman’s correlation analysis confirmed the correlation between circ_0087429 and OGN expression in cervical cancer tissue samples. **p < 0.01, ***p < 0.001, NS means no statistical significance