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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression

Fig. 8

EIF4A3-regulated circ_0087429 can inhibit EMT in cervical cancer cells through the circ_0087429/miR-5003-3p/OGN axis. a-b. Immunofluorescence staining detected the fluorescence intensities of N-cadherin or E-cadherin upon circ_0087429 overexpression or knockdown, respectively. Scale bar, 25 μm. c-d. EMT related proteins in each group of cells were detected by western blotting. e. The binding sites for EIF4A3 in the flanking sequences of the SPIN1 mRNA transcript were predicted using CircInteractome. f. RIP assay demonstrated the binding between SPIN1 and EIF4A3. g. The differential expression of EIF4A3 between cervical cancer and normal cervical epithelial cell lines was detected by western blotting. h-i. The expression of EIF4A3 in the two groups of SiHa cells of oe-NC and oe-EIF4A3 was detected by qRT-PCR and western blotting. j-k. The expression of EIF4A3 in Caski cells treated with siRNA was detected by qRT-PCR and western blotting. l. The expression of circ_0087429 and EIF4A3 in SiHa cells stably overexpressing EIF4A3 were detected by qRT-PCR. m. The expression of circ_0087429 and EIF4A3 in Caski cells treated with si-EIF4A3 were detected by qRT-PCR. *p < 0.05, **p < 0.01, ****p < 0.0001

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