Fig. 5

HEATR5B-881aa, but not circHEATR5B, exerted inhibitory effects on GBM cells. A HEATR5B protein and HEATR5B-881aa were detected by western blot assays in U251 and U373 cells transfected with OV-HEATR5B-881aa or circHEATR5B-ATG-Mut plasmid. B and C The effects of HEATR5B-881aa on glucose consumption (B) and lactate production (C) in U251 and U373 cells. D The effects of HEATR5B-881aa on ECAR in U251 and U373 cells. E The effects of HEATR5B-881aa on proliferation in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). n.s., ^***P < 0.001 vs. OV-NC group by one-way ANOVA. F HEATR5B protein and HEATR5B-881aa were detected by western blot assays in U251 and U373 cells transfected with sh-circHEATR5B plus OV-HEATR5B-881aa plasmids. G and H The effects of circHEATR5B and HEATR5B-881aa on glucose consumption (G) and lactate production (H) in U251 and U373 cells. I The effects of circHEATR5B and HEATR5B-881aa on ECAR in U251 and U373 cells. J The effects of circHEATR5B and HEATR5B-881aa on proliferation in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^***P < 0.001 vs. sh-circHEATR5B + OV-HEATR5B-881aa-NC group by one-way ANOVA