Fig. 1
IL-6 and Sox4 were memorably up-regulated, and positively correlated in OSCC tissues. (A) RT-qPCR analysis of IL-6 in normal, OLP, and OSCC tissues. (B) The content of IL-6 was tested with ELISA kit in the serum of normal, OLP, and OSCC patients. (C) IL-6 expression was determined via IHC in normal, OLP, and OSCC tissues. (D and E) Western blotting analysis of IL-6 in normal, OLP, and OSCC (developed after follow-up) tissues, which were from the same patient (n = 9). After diagnosis of OLP, OLP tissue and adjacent normal tissue were taken, and OSCC tissue was taken after follow-up. (F) The overall survival of IL-6 expression in OSCC patients. (H) The level of Sox4 was confirmed via RT-qPCR. (H and I) Sox4 expression levels were assessed with Western blot in normal, OLP, and OSCC tissues, which also were from the same patient (n = 9). (L) The overall survival of Sox4 expression in OSCC patients. (J) Immunohistochemical staining of Sox4 expression in normal, OLP, and OSCC tissues. (K) Correlation analysis of IL-6 and Sox4 (r = 0.8226). ** P < 0.01, *** P < 0.001, **** P < 0.0001