Fig. 5

TEAD4 enhances the metastatic and invasion potential of BLCA cells (A) Relative protein expression of TEAD4 in normal bladder cell SV-HUC1 and four BLCA cell lines, as detected by immunoblot analysis. (B, C) RT-qPCR and western blot analysis of TEAD4 expression level in the BLCA cells transfected with LV-NC or LV-shTEAD4. (D, E) rt-PCR and western blot analysis of TEAD4 expression level in the BLCA cells transfected with Vector or OE-TEAD4 plasmid. (F) Representative data from Transwell migration and Matrigel invasion assays performed with the TEAD4 knock down cells (T24, 2 × 10⁴ cells, 24 h; 5637, 4 × 10⁴ cells, 36 h). (G) Representative data from Transwell migration and Matrigel invasion assays performed with the TEAD4 overexpression cells (5637, 4 × 10⁴ cells, 24 h; TCCSUP, 4 × 10⁴ cells, 36 h). (H) Representative data from wound healing migration assays performed with the TEAD4 knock down cells. (I) Representative data from wound healing migration assays performed with the TEAD4 overexpression cells. Data are expressed as mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001)