Fig. 4

circTOLLIP serves as a sponge for miR-516a-5p. a RIP was performed with an anti-AGO2 antibody in 97H cells. b-c Relative circTOLLIP and microRNA enrichment using biotinylated control probe and specific circTOLLIP probes in 97H cells. d Relative luciferase reporter activity of WT-circTOLLIP after transfection with mimics of the most 4 most enriched microRNAs in HEK-293 T cells. e Schematic view of miR-516a-5p putative binding sites with circTOLLIP and construction of correspondent mutant circTOLLIP reporter plasmid. f Luciferase reporter activity of circTOLLIP in 97H cells co-transfected with miR-516a-5p mimic or NC mimic. g RIP was performed using an anti-AGO2 antibody in 97H cells transfected with miR-516a-5p mimic or NC mimic. h circTOLLIP enrichment using biotinylated NC probe and miR-516a-5p probes in 97H cells. i Colocalization between cicTOLLIP and miR-516a-5p was observed by FISH assay. Scale bars = 50 μm