Fig. 5

miR-516a-5p inhibits the proliferation and metastasis of HCC cells in vitro and in vivo. a CCK-8, cell colony formation assay (b), scratch wound healing assay (c), and cell migration and invasion assays (d, e) were performed in HLF and 97H cells transfected with miR-516a-5p mimic or NC mimic. f The subcutaneous xenograft tumors from nude mice that transplanted with stablely overexpressing miR-516a-5p and vector 97H cells. g qRT–PCR analysis of miR-516a-5p level in xenograft tumors and quantification of tumor volume (h) and weight (i). j Livers from nude mice orthotopically transplanted with miR-516a-5p-overexpressing and vector 97H cells. Representative images showing the livers from the nude mice and HE staining of isolated liver tissues. The miR-516a-5p expression was analyzed in two groups (k) and the number of visible tumor nodules was counted (l). m-n The relative miR-516a-5p levels in 52 paired HCC and adjacent nontumor tissues. o The correlation between circTOLLIP and miR-516a-5p in HCC tissues. p Kaplan–Meier analysis of the correlation between miR-516a-5p expression and OS in HCC according to data from Kaplan–Meier Plotter database. Low miR-516a-5p group: n = 87, high miR-516a-5p group: n = 79