Fig. 1

Circ_0004585 is upregulated in PCa tissues and cell lines, and mainly distributes in the cytoplasm. A The The Venn diagram screens out three CEMIP cyclization products. B qRT-PCR assay detect the expression level of circ_0004585 in anoikis-resistant PCa cells (PC-3-AR、DU145-AR) and the corresponding parent cells (PC-3-P、DU145-P). C qRT-PCR assay detect the expression level of circ_0004585 in 10 pairs of PCa and adjacent non-tumor tissue. D The schematic diagrams show that thirteen exons derived from CEMIP constitute circ_0004585. The existence of circ_0004585 was demonstrated by PCR and its back-splicing junction was verified by Sanger sequencing. E PCR assay with divergent or convergent primers indicating circ_0004585 is present in the PC-3 and DU145 cell lines. GAPDH was used as a negative control. F Real-time qPCR analysis of the expression of circ_0004585 after RNase R treatment in PC-3 and DU145 cells. G RNA-FISH indicates the location of circ_0004585. Nuclei were stained blue with DAPI. Circ_0004585 is stained red with Cy3 (Scale bar, 10 μm). Bar graphs show the statistical analysis of three independent experiments (* P < 0.05; ** P < 0.01)