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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells

Fig. 5

Overexpression of TM9SF4 is correlated with unfavourable pathological characteristics in PCa. A, B TM9SF4 expression in TCGA PRAD and GSE6956 dataset. C Disease-special survival in the TCGA PRAD dataset with low versus high levels of TM9SF4 mRNA, The cut-off value of TM9SF4 was used to distinguish between low expression (less than the cut-off value) and high expression (greater than or equal to the cut-off value). D TM9SF4 expression in 60 paired paraffin-embedded PCa and adjacent tissues detected by immunohistochemical staining (Original magnification, × 400). E Schematic representation of the H-score of TM9SF4 expression. F-H Western blotting assay showed the TM9SF4 protein expression in 10 pairs of PCa tissues, prostatic epithelial cells (RWPE-1), several PCa cell lines (22RV1, PC-3, DU145, LNCaP, C4–2), and anoikis-resisrant and corresponding parental PCa cells (PC-3, DU145). I Correlation of TM9SF4 and circ_0004585 expression in 10 pairs of PCa tissues. J The protein levels of TM9SF4 detected by western-blotting in circ_0004585 combined with miR-1248 overexpressing PC-3-AR and DU145-AR cells. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05; ** P < 0.01)

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