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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: GABPA-activated TGFBR2 transcription inhibits aggressiveness but is epigenetically erased by oncometabolites in renal cell carcinoma

Fig. 2

GABPA regulates proliferation, stemness and invasion of ccRCC cells. A GABPA-specific siRNAs and its expression vectors efficiently inhibit and overexpress GABPA in A498 and 786-O cells, respectively, as determined using immunoblotting. B GABPA depletion promotes while its overexpression inhibits cell proliferation. IncuCyte S3 Live-Cell Analysis System was employed to measure cell proliferation rate. C GABPA depletion increases while its overexpression reduces cells at S phase, as assessed using flow cytometry. D and E GABPA depletion increases while its overexpression reduces the stemness of A498 and 786-O cells. Scale bars: 200 µm. F and G GABPA depletion increases while its overexpression reduces the invasive capacity of A498 and 786-O cells. Scale bars: 100 µm. (H-J) GABPA regulates the expression of proliferation-, stemness- and EMT-related genes. (K) ccRCC stem cell (CD44 and CD105) and mesenchymal stem cell markers (CD90) are upregulated in GABPA-depleted cells. Three independent experiments were performed. *, ** and *** denote P < 0.05, 0.01 and 0.001, respectively

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