Fig. 4

GABPA activates the TGFβ pathway to exert its effects on ccRCC cells. A and B The accelerated cell proliferation resulting from GABPA knockdown is abolished by TGFBR2 overexpression. C The enhanced cell invasion resulting from GABPA knockdown is abolished by TGFBR2 overexpression. Scale bars: 100 µm. D TGFβ-mediated SMAD2/3 phosphorylation is abolished by knockdown of either GABPA or TGFBR2. E The inhibition of cell proliferation mediated by TGFβ is significantly attenuated by knockdown of GABPA or TGFBR2. F The inhibition of cell invasion mediated by TGFβ is significantly attenuated by knockdown of either GABPA or TGFBR2. Scale bars: 100 µm. G The stemness or self-renewal inhibition mediated by TGFβ is abolished by knockdown of either GABPA or TGFBR2. Scale bars: 200 µm. H The schematic illustration for TGFβ and cMYC targets. I TGFβ-mediated downregulation of cMYC is abolished by knockdown of either GABPA or TGFBR2. J The expression of TGFβ and/or cMYC target genes are altered in response to TGFβ treatment and knockdown of GABPA or TGFBR2. Three independent experiments were performed. *, ** and *** denote P < 0.05, 0.01 and 0.001, respectively