Fig. 1

DHX9 is associated with poor prognosis in PC patients. A Expression profiling of DHX9 mRNAs in human normal and tumor specimens from Gene Expression Omnibus profile dataset (GSE29079). Statistical analysis was performed by Student’s t test and significance is indicated in the plot. B Boxplot represents DHX9 expression in PC patients grouped according to the different Gleason score (TCGA dataset: Tumor Prostate Adenocarcinoma-TCGA-rsem-tcgars). C Expression profiling of DHX9 mRNAs in human tumor specimens from stable and progressive disease, with complete or partial remission, from TCGA dataset (Tumor Prostate Adenocarcinoma-TCGA-rsem-tcgars) plotted for biochemical recurrence. Statistical analysis was performed by Student’s t test and significance is indicated in the plot. D Kaplan-Meier curve of Disease-Free Survival rate of PC patients analysed for DHX9 expression (492 patients, TCGA dataset). The blue line shows patients with low DHX9 expression, the red line shows patients with high DHX9 expression. Significance is indicated in the plot. LNCaP cells were transfected with a control siRNA (siCTRL) or a siRNA specific for DHX9 (siDHX9) and analyzed by WB after 48 hrs (E) and (F) MTS assay after 24, 48, and 72 hours. Values are the mean ± SD of three independent experiments, each performed in triplicate, considering the siCTRL at 24 hrs as 1. (G) siCTRL and siDHX9 LNCaP cells were transfected and cultured for 48 hrs as in (E) and migration assay was performed. The crystal violet–stained migrating cells were photographed (left) and counted (right). Values are the mean ± SD of three independent experiments, considering the siCTRL as 100. Magnification, × 10. H Colony assay was performed in LNCaP cells transfected with either control (siCTRL) or DHX9 siRNA (siDHX9). Histogram represents the percentage of colonies, reported as the mean ± SD of three experiments, considering the siCTRL as 100. Statistical analysis in (F) was performed by two-way ANOVA and in (G) and (H) was performed by Student’s t test, p values: *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001