Fig. 2

DHX9 depletion affects gene expression in LNCaP cells. A Schematic representation of the distribution of up and down regulated genes upon DHX9 silencing. B Gene Ontology (performed using DAVID) of terms regulated at gene expression levels by analyzing DEGs after DHX9 silencing. Histograms represent the Fold Enrichment score and the -log₁₀ (p-values). C Heatmap summarizing the outcome of the RNA sequencing for the genes selected for RT-qPCR validations. D Validation of DEGs regulated by DHX9 silencing was performed by RT-qPCR analyses, using the indicated primers. Values are the mean ± SD of three independent experiments, considering the siCTRL as 1. Statistical analyses were performed by Student’s t test, p values: ***, p ≤ 0.001