Fig. 3

DEGs upon DHX9 silencing are enriched in AR targets. A Gene Set Enrichment analysis (GSEA) of androgen response gene signature in siDHX9 versus siCTRL LNCaP cells. Androgen response resulted as the second most significant category after mTORC signaling (Suppl. Table S3). Bars represent individual genes in a ranked data set list. NES, normalized enrichment score. B Gene set enrichment analysis (performed via EnrichR) of DEGs after siDHX9. The overlap between the two analysis is shown in the Venn diagram (p-value = 0.001) in (C). D DEGs after DHX9 silencing were overlapped with DEGs in response to Enzalutamide treatment (p-value < 0.0001). E qPCR analyses of ChIP experiments performed in LNCaP cells using AR antibody and anti-IgG rabbit Abs (IgG). Associated DNA was expressed as % of input. Values are the mean ± SD of three independent experiments. Statistical analysis was performed by Student’s t test, p values: *, p ≤ 0.05; **, p ≤ 0.01; ****, p ≤ 0.0001