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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: The DNA/RNA helicase DHX9 contributes to the transcriptional program of the androgen receptor in prostate cancer

Fig. 5

DHX9 interacts with AR and is required for the AR recruitment on the promoter of its target genes. A LNCaP cells were transfected with either control siRNA (siCTRL) or a siRNA specific for DHX9 (siDHX9) for 48 hrs. DHX9 and AR expression was measured by WB analysis. Histogram represents the relative AR protein expression versus siCTRL, normalized to GAPDH expression. B Cytosolic (C) and nuclear (N) LNCaP extracts were immunoprecipitated with AR antibody (IP AR). IP and input were subjected to WB to analyse the expression of DHX9 and AR. GAPDH and H3 were used as cytosolic and nuclear markers. C LNCaP cells were transfected with a control siRNA (siCTRL) or a siRNA specific for DHX9 (siDHX9) for 48 hours and ChIP experiments were performed using AR antibody. Associated DNA was measured by qPCR and histogram represents the fold over CTRL, expressed as % of input. Statistical analysis was performed by Student’s t test, p values: ***, p ≤ 0.001

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