Fig. 1

a A Multiplexed FISH strategy, achieves spatial information retention of the cell transcriptome through multiple rounds of FISH hybridization and binary computation. b In SPBC, tissues are placed on slides lined with beads containing gene probes containing positional information that can bind to tissue RNA. The tissue RNA is permeabilized onto the slide by the action of enzymes, and the tissue RNA that has bound to the probe is subsequently reverse transcribed into cDNA and sequenced