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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: New molecular mechanisms in cholangiocarcinoma: signals triggering interleukin-6 production in tumor cells and KRAS co-opted epigenetic mediators driving metabolic reprogramming

Fig. 4

EGFR-KRAS signaling triggers IL6 expression in CCA cells. a Effect of HB-EGF on IL6 mRNA expression (12 h treatment) and IL6 protein release (24 h treatment) in HuCCT-1 and Huh28 cells. *p < 0.05, **p < 0.01. b Characterization of control and KRASG12D (MutKRAS) Huh28 cells. Images show representative western blot analyses of KRASG12D, p-MEK1/2, p-ERK1/2, p-STAT3, STAT3 levels, as well as GAPDH levels, as loading control, in both cell lines. c Expression levels of IL6 mRNA and IL6 protein concentrations in the conditioned media (24 h culture) of control and MutKRAS Huh28 cells. **p < 0.01. d Expression levels of IL6 mRNA in MutKRAS cells treated with PI3K (LY294002) or MEK (PD98059) inhibitors for 6 h. *p < 0.05

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