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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Depleting receptor tyrosine kinases EGFR and HER2 overcomes resistance to EGFR inhibitors in colorectal cancer

Fig. 1

PEPDG278D inhibits CRC cells resistant to cetuximab. a Western blotting of untreated whole cell lysates. b Effects of PEPDG278D and cetuximab on cell growth measured by MTT assay. Each value is mean ± SD (n = 3). ****P < 0.0001 by one-way ANOVA, followed by Tukey test for comparison with the control. c Western blotting of whole cell lysates after treatment of the cells with vehicle, PEPDG278D (25 nM), or cetuximab (275 nM) for 48 h. The following phosphorylation sites were measured: pY1173-EGFR, pY1221/1222-HER2, pY1328-HER3, pY1234/1235-MET, pY1131-IGF1R, pY416-SRC, pS473-AKT, and pT202/Y204-ERK. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was measured as a loading control here and elsewhere. HCT116 cell lysates were used as a positive control for measurement of p-SRC and p-AKT in SW48 cells and for measurement of EGFR and HER2 in SW620 cells

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