Fig. 3

STAT3 regulates ATG7-dependent autophagy in icotinib resistant cells. A Immunoblotting analysis of changes induced by icotinib in STAT3 activation and FOXM1 levels in indicated cells. B-D Detection of protein changes in STAT3 activity, FOXM1 and ATG7 levels (B), autophagic flux with the mRFP-GFP-LC3 reporter (C), and cell apoptosis (D), induced by icotinib in indicated cells with or without the treatments of IL-6 (10 ng/ml/24 h) or CTN (7 μM/24 h). E-G In the indicated cells with or without icotinib treatment, protein changes in STAT3 activation, FOXM1 and ATG7 levels (E), the autophagic flux with the mRFP-GFP-LC3 reporter (F), and apoptosis (G) in the absence and presence of STAT3WT, STAT3-CA or STAT3-Y705F plasmids, are shown. [**P < 0.01, ***P < 0.001, ns, no significance as compared with the DMSO group. ##P < 0.01, ###P < 0.001, NS, no significance as compared with icotinib group. §§P < 0.01, §§§P < 0.001, Ns, no significance as compared with the combination group of icotinib and STAT3WT. nS as compared with the group of STAT3WT]