Fig. 5
circPDE5A regulates the m6A modification of EIF3C mRNA thorough WTAP. A, MeRIP assay showing the m6A modification level of EIF3C with WTAP knockdown in C4-2B and 22Rv-1 cells. B, MeRIP assay showing the m6A modification level of EIF3C with WTAP overexpression in C4-2B and 22Rv-1 cells. C, WTAP-RIP assay showing the binding capacity between EIF3C mRNA and WTAP with circPDE5A knockdown in C4-2B and 22Rv-1 cells. D, WTAP-RIP assay showing the binding capacity between EIF3C mRNA and WTAP with circPDE5A overexpression in C4-2B and 22Rv-1 cells. E, Western blotting assay showing the protein levels of EIF3C and WTAP in circPDE5A overexpression C4-2B and 22Rv-1 cells with the overexpression of WTAP. F, G, YTHDF1-RIP assay showing the binding capacity between YTHDF1 and EIF3C mRNA in 22Rv-1 cells with circPDE5A knockdown (F) or overexpression (G). H, Western blotting assay showing the EIF3C and YTHDF1 protein expression in circPDE5A knockdown C4-2B and 22Rv-1 cells with the silencing of YTHDF1. Data represents mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001