Fig. 6

EIF3C promotes prostate cancer cells metastasis through the MAPK pathway. A, The relative mRNA expression level of EIF3C in PCa and normal specimens obtained from the TCGA database. B, RT-qPCR showing the relative mRNA expression level of EIF3C in our PCa cohort. C, Western blotting assay showing the protein expression level of EIF3C in 15 paired PCa and normal specimen. D, Quantification of the band intensities during Western blot. E, Transwell assay in C4-2B and 22Rv-1 cells transfected with EIF3C siRNAs or the siRNA control. Scale bars, 5 μm. F, Transwell assay in C4-2B and 22Rv-1 cells transfected with EIF3C overexpression plasmids or vector. Scale bars, 5 μm. G, H, Western blotting showing the effect of EIF3C overexpression (G) or knockdown (H) on the MAPK pathway proteins in C4-2B and 22Rv-1 cells. Data represents mean ± S.D. from three independent experiments. ***, p < 0.001