Fig. 7

circPDE5A restrains prostate cancer metastasis via EIF3C. A, B, Western blotting showing the effect of circPDE5A overexpression (A) or knockdown (B) on the MAPK pathway proteins. C, Western blotting showing the EIF3C protein expression in circPDE5A overexpression C4-2B or 22Rv-1 cells with the overexpression of EIF3C. D, Transwell assay showing the migration and invasion ability of 22Rv-1 cells described in (C). Scale bars, 5 μm. E, Western blotting showing the EIF3C protein expression in circPDE5A knockdown C4-2B or 22Rv-1 cells with the silencing of EIF3C. F, Transwell assay showing the migration and invasion ability of 22Rv-1 cells described in (E). Scale bars, 5 μm. G, Western blotting assay showing the effect of EIF3C overexpression on the MAPK pathway proteins in C4-2B or 22Rv-1 cells described in (C). H, Western blotting assay showing the effect of EIF3C knockdown on the MAPK pathway proteins in C4-2B or 22Rv-1 cells described in (F). Data represents mean ± S.D. from three independent experiments. **, p < 0.01; ***, p < 0.001