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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Fast detection, a precise and sensitive diagnostic agent for breast cancer

Fig. 2

Nanoprobe highlight tumor cells nucleus through ATP-dependent endocytosis. A Schematic illustration for the process of nanoprobe entered into nucleus by endocytosis. B The cellular localization of AS1411@RuPEP (5 µM) in MDA-MB-231 cells. The enlarge and 3D tomoscan image of the MDA-MB-231 nucleus in the presence of AS1411@RuPEP. The transport pathway of cellular uptake for complex AS1411@RuPEP in MDA-MB-231 cells. C Real-time imaging of the MDA-MB-231 cells treated with complex AS1411@RuPEP (5 μM) for 2 h. The cell morphology was captured using phosphorescence microscopy every 15 min. D Celluar uptake of AS1411@RuPEP (5 μM) was incubated in MDA-MB-231 cells labeled cell membrane in green fluorescence by DiO for 6 h at 37 °C and 4 °C; MDA-MB-231 cells cells were pre-treatment with Chlorpromazine (6 nM); Dexy (10 nM) + Olig (5 nM), CCCP (10 nM), respectively, then replacement of the inhibitors with AS1411@RuPEP (5 μM) was incubated in MDA-MB-231 cells for 6 h at 37 °C. E Bio-TEM imaging of MDA-MB-231 cells for cellular uptake of AS1411@RuPEP. Cells are treated with AS1411@RuPEP for 6 h at 37 °C

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