Fig. 4

Specific-targeted NIR fluorescence tumor imaging intransgenic MMTV-PyMT primacy breast cancer mice. A The arrows show the tumor sites. Time-dependent in vivo NIR fluorescence images of transgenic MMTV-PyMT primacy breast cancer mice after tail intravenous injection of 20 μM, 100 μL, AS1411@RuPEP. (Excited by 475 nm, emission at 680 nm). B Fluorescence intensity of AS1411@RuPEP in mice tumor area and non-tumor area is quantitatively determined. C Fluorescence intensity of AS1411@RuPEP (average cps) in dissected organs or tissues is quantitatively determined. Data are presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. D Tissue distribution and drug metabolism of AS1411@RuPEP at 24, 72 and 108 h. E Histochemistry analysis of heart, liver, spleen, lung, kidney and brain section stained with hematoxylin eosin of transgenic MMTV-PyMT primacy breast cancer mice at 108 h after i.v. administration of saline and 20 μM. Bar: 50 μm