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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Fast detection, a precise and sensitive diagnostic agent for breast cancer

Fig. 5

Distinguish and detect tumor area in human breast cancer patient section for nanoprobe. A Histochemistry analysis of human breast cancer tissues section stained with hematoxylin eosin of five specimens of invasive ductal carcinoma patients. B The targeting imaging of nanoprobe (5 μM) to distinguish cancerous area and paracancerous area in the frozen sections (OCT-embedded) of the fresh human invasive ductal carcinoma tissues observed by fluorescence microscope. C CLSM enlarged observation of the distribution of nucleolin and nanoprobe in cancerous area and paracancerous area. The whole tissues were stained by DAPI in blue, NCL stained in Green and Nanoprobe is red. D The merge curve of three channels emission intensity for cancerous and paracancerous cells analyzed by using Image-Pro Plus softwire. E The NCL protein expression of cancerous and paracancerous tissues in five specimens of invasive ductal carcinoma patients (n = 5). F Quantitative analysis of NCL expression and tumor grade of five specimens of invasive ductal carcinoma specimens (n = 5). Note: The fresh human cancer tissues were The data are presented as the means ± SDs of three independent experiments. *P < 0.05,** P < 0.01 and ns—not significant

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