Table 1 Primary antibodies used for immunohistochemistry (IHC) and Western blot analysis (WB)
Antibody name | Use | Concentration | Company | Catalogue No. |
|---|---|---|---|---|
CK19 | IHC | 1:400 | Abcam | Ab133496 |
CK7 | IHC | 1:400 | Abcam | Ab181598 |
HNF4α | IHC | 1:100 | Abcam | Ab181604 |
HNF1B | IHC | 1:50 | Proteintech | 12533-1-AP |
Periostin | IHC | 1:100 | Abcam | ab215199 |
Osteopontin | IHC | 1;100 | Abcam | ab283656 |
Hydroxyproline | IHC | 1:100 | Cell Signaling Technology | 73812 |
SOX9 | IHC | 1:500 | Cell Signaling Technology | 82630 |
Vimentin | IHC | 1:1000 | Abcam | ab92547 |
Phospho-TAZSer89 | IHC | 1:100 | Cell Signaling Technology | 59971 |
V5-tag | IHC | 1:100 | Abcam | ab95038 |
Yap | IHC; WB | 1:100; 1:1000 | Cell Signaling Technology | 14074 |
α-SMA | IHC | 1:400 | Abcam | ab5994 |
TAZ/WWTR1 | IHC | 1:100 | Sigma-Aldrich | AMab90730 |
TAZ | IHC | 1:200 | Cell Signaling Technology | 72804 |
Yap/Taz | WB | 1:1000 | Cell Signaling Technology | 8418 |
Notch1 | IHC | 1:100 | Cell Signaling Technology | 3608 |
Notch2 | IHC | 1:50 | Cell Signaling Technology | 5732 |
JAGGED1 | IHC | 1:100 | Cell Signaling Technology | 70109 |
Phospho-AKTSer473 | IHC | 1:250 | Cell Signaling Technology | 4060 |
Phospho-GSK-3βSer9 | IHC | 1:200 | Cell Signaling Technology | 9323 |
Phospho-RPS6Ser235/236 | IHC | 1:400 | Cell Signaling Technology | 4856 |
FOXA1 | IHC | 1:400 | Abcam | ab170933 |
FOXA2 | IHC | 1:200 | Abcam | ab108422 |
CEBPA | IHC | 1:100 | Cell Signaling Technology | 8178 |
CPS1 | IHC | 1:200 | Abcam | ab129076 |
CYP2E1 | IHC | 1:200 | Proteintech | 19937-I-AP |
CYP3A4 | IHC | 1:400 | Proteintech | 18227-1-AP |
ARG1 | IHC | 1:500 | Abcam | ab233548 |
GLUL | IHC | 1:500 | BD Biosciences | 610517 |
CD44v6 | IHC | 1:100 | ThermoFischer Scientific | MA1-81995 |
CD133 | IHC | 1:100 | Abcam | ab271092 |
EPCAM | IHC | 1:100 | Abcam | ab221552 |
CD34 | IHC | 1:150 | Abcam | Ab8158 |
Podoplanin | IHC | 1:200 | Abcam | ab256559 |
NCAM1/CD56 | IHC | 1:400 | Cell Signaling Technology | 99746 |
Ki67 | IHC | 1:500 | Bethyl Laboratories | IHC-00375 |
S100A4 | IHC | 1:100 | Cell Signaling Technology | 13018 |
PDGFRβ | IHC | 1:100 | Cell Signaling Technology | 4564 |
Phospho-LATS1/2T1041/1079 | IHC | 1:100 | Abcam | 11344 |
Cleaved Caspase 3 | IHC | 1:50 | Cell Signaling Technology | 9661 |
Cleaved PARP | IHC | 1:100 | Cell Signaling Technology | 94885 |
CD4 | IHC | 1:100 | Cell Signaling Technology | 25229 |
CD45 | IHC | 1:100 | Cell Signaling Technology | 70257 |
F4/80 | IHC | 1:200 | Cell Signaling Technology | 70076 |
β-Actin | WB | 1:5000 | Santa Cruz Biotechnology | sc-10731 |