Fig. 1
Expression of GR reduced proliferation, induced neuronal and glial differentiation, and decreased tumor growth. A Immunofluorescence staining of BE(2)-EV and BE(2)-GR cells after ligand treatment. Green = SCG2; red = Phalloidin; blue = DAPI. B Western blot of indicated markers in BE(2)-EV and BE(2)-GR cells following incubation with ligands. Please note the different loading order on the two membranes. C RT-qPCR of NEFL and SOX2 in BE(2)-EV and BE(2)-GR cells following ligand treatment. B2M was used as housekeeping gene. Statistical analysis: t-test with *, **, ***, and **** indicating p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively. Data of the fold mRNA expression is presented as mean ± SD. D Immunofluorescence staining of SH-SY5Y parental and GR expressing cells after incubation with ligands. Green = SCG2; red = Phalloidin; and blue = DAPI. E Western blot of neural differentiation markers in SH-SY5Y parental and GR expressing cells following ligand treatment. F Immunofluorescence staining of SK-N-AS parental and GR expressing cells after incubation with ligands. Green = Vimentin; red = Phalloidin; blue = DAPI. G Western blot of neural and glial differentiation markers in SK-N-AS parental and GR expressing cells following ligand treatment. All experiments were carried out during seven days. Ethanol was used as control for DEX, DMSO for ATRA, and ethanol + DMSO for the combination. Scale bars represent 20 μm. β-actin was used as loading control and molecular weight markers are shown to the left. All results are representative of at least three independent experiments